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WORD: After the coupling of the primary C-terminal amino acid of all peptides to the amino group of the membrane, the amino groups between the spots and also some of the amino teams within the spot areas don't kind bonds with the amino acids. OBSERVE: This avoids the coupling of amino acids within the additional cycles with the membrane as a substitute of the growing peptide chain. Be sure that all the purification buffers are at four °C and perform the protein purification within the chilly room to keep away from protein denaturation.

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